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Record the salt form, solvent, concentration basis, preparation date, operator, and storage condition

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Nrf2 deficiency in aged mice exacerbates cellular senescence promoting cerebrovascular inflammation

(7) These are observational cohorts with their own populations, confounders, and limits, so the numbers describe an association in a group, not a prediction for an individual

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR