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Quantitative PCR (qPCR) was performed using a Strategene Mx3005P qPCR system (Santa Clara, CA), and a CT analysis was performed using basal bEnd.3 cells as controls

The amyloidogenic core sequence of hIAPP (residues 2029, known as the FGAILS region) has been substituted to eliminate -sheet aggregation propensitya critical requirement for generating a stable, injectable research compound

Monitor for mild injection-site redness or systemic effects like headache

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This stability eliminates the need for protective carriers that other growth factors require