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coli OP50 was cultured in 50 ml of LB broth overnight at 37C

This minimizes common symptoms like nausea and diarrhea

Eating smaller, more frequent meals may help reduce gastrointestinal side effects

Guanosine and uroguanosine, which target guanosine cyclase (GC-C) receptors in the intestine and kidney, lead to elevated intracellular cGMP concentrations, and high GC-C expression in L cells is associated with regulation of GLP-1 release (Friedlander et al., 2011)

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