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After purification, islets were cultured overnight at 37 C in a humidified atmosphere of 95% air and 5% CO 2 in RPMI 1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 0.1 mg/ml streptomycin

G., Carli, F., Sabatini, S., Astiarraga, B., Ferrannini, E., Camastra, S

PubMed Central , Accessed 17 Feb

Patients should confirm they have reached their target maintenance dose and have been at that dose for at least 46 weeks before concluding that appetite suppression is inadequate

They stimulate insulin secretion, inhibit glucagon release, and slow gastric emptying, which aids in lowering blood sugar levels and promotes satiety