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Western blot (WB) results revealed a significant increase in the expression of Ki-67 and Lamin B, while the protein levels of P21, P16, and -H2AX decreased following FOXO4-DRI treatment (Figure 4A)
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Detection indicators: bone density (lumbar spine, hip), bone turnover markers (OC, CTX), bone microstructure (number, thickness, and gaps of bone trabeculae), and skeletal mechanical strength
This was the approach reported in several key studies with gilthead seabream and European seabass (Schrama et al., 2018
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