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Neurons were incubated in Hams F12 medium containing 10% heat inactivated fetal calf serum and antibiotics (100 g/ml each penicillin and streptomycin), with added neurotrophic factors (NGF-100 ng/ml, GDNF and NT3-50 ng/ml), in collagen (50 g/ml) and laminin (20 g/ml) coated glass bottom plastic petri dishes (MatTek Corp
10.1038/NATURE11729 74 HigginsC
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