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To comprehensively evaluate the therapeutic effects of semaglutide on hepatic steatosis, future studies should incorporate complementary methods such as Oil Red O staining (Zhu et al., 2019), biochemical hepatic TG assays (Rong et al., 2024), or non-invasive imaging techniques such as magnetic resonance imaging with proton density fat fraction (MRI-PDFF) (Park et al., 2022)

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When B12 levels are equivocal, additional testing with methylmalonic acid (MMA) and homocysteine levels can provide greater diagnostic accuracy, as both are elevated in true B12 deficiency

For MCAS, elevated baseline serum tryptase or urinary histamine metabolites may be helpful

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