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The subcutaneous route is still used, and it has some practical advantages
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(4)Fmoc-Ala-Gly-Leu-Val-WangResin (4) Preparation of Fmoc-Ala-Gly-Leu-Val-WangResin (3)Fmoc-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Ala-OH0.7gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.5 3DMF3DMF2Fmoc-Ala-Gly-Leu-Val-WangResin In the Fmoc-Gly-Leu-Val-WangResin of step (3), add decapping reagent (20% piperidine in DMF solution), react at room temperature for 5 minutes, drain, add decapping reagent at normal temperature and react for 20 minutes, and use isopropyl Wash 3 times with alcohol, 3 times with DMF, 2 times with distilled DMF, drain, add Fmoc-Ala-OH0.7g, DIEA0.4ml, TBTU0.56g, HOBT0.2g, DMF10ml, react at room temperature for 1.5 hours, use Vacuum pump to dry, wash 3 times with isopropanol, DMF wash 3 times, DMF washed twice, dry, obtain Fmoc-Ala-Gly-Leu-Val-WangResin
Run the water slowly down the vial wall, not directly onto the powder
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