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The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)

So, while we dont get much B12 in the water anymore, we dont get much cholera, either, which is a good thing

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For easier dosing calculations, many practitioners use: 2 mg vial + 2 mL bacteriostatic water = 1 mg/mL (1,000 mcg/mL) 5 mg vial + 2 mL bacteriostatic water = 2.5 mg/mL (2,500 mcg/mL) Step 3: Draw Bacteriostatic Water Clean the rubber stopper of the bacteriostatic water vial with an alcohol prep pad