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After blocking with 5% BSA in TBST, slides were stained with rat anti-BrdU antibody (which detects CldU but not IdU, OBT0030, 1:2000, AbDSerotec) and mouse anti-BrdU antibody (which detects IdU but not CldU, 7580, 1:1000, BD Biosciences) at 4 C for overnight
PubMed Garvey, W.T., Batterham, R.L., Bhatta, M., Buscemi, S., Christensen, L.N., Frias, J.P., et al
bioRxiv (2020)
GLP-1s: What They Do (and Dont Do) How they work: GLP-1 receptor agonists mimic a gut hormone that slows gastric emptying, increases satiety, and regulates blood sugar
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