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For short term treatments, seedlings at the age of 3 days post germination (DPG), were transferred, in darkness, to liquid MS supplemented with 20 nM, 40 nM methotrexate (MTX) or with 0.4% dimethylsulfoxide (DMSO) as a control

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Since the pancreas has abundant GAD, a direct immunological destruction occurs in the pancreas and the patients will have diabetes mellitus