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Culture media consisted of 10 g/L glucose, 1 g/L yeast extract, 5 g/L (NH 4 ) 2 SO 4 , 2 g/L KH 2 PO 4 , 0.5 g/L MgSO 4 7H 2 O, 0.1 g/L CaCl 2 2H 2 O, 0.02 g/L FeSO 4 7H 2 O, 0.01 g/L ZnSO 4 7H 2 O, 0.005 g/L CuSO 4 5H 2 O, 0.001 g/L MnCl 2 4H 2 O, 2.5 mL of 70% H 2 SO 4 and 0.5% (v/v) Antifoam 204 (Sigma Aldrich, St

Denson LA, Kim MO, Bezold R, Carey R, Osuntokun B, Nylund C, Willson T, Bonkowski E, Li D, Ballard E, Collins M, Moyer MS, Klein DJ

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Syringe volume Match your syringe volume to your dose