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For GSH assay, cells were treated in Earles balanced salt solution (EBSS), supplemented with 25 mM glucose, 4 mM glutamine, 1 X MEM vitamin solution (Life technologies, CA, USA), 0.22% wt/vol NaHCO 3 , 1 mM sodium pyruvate, 0.2% BSA, 25 mM HEPES, 100 U/ml penicillin and 100 g/ml streptomycin in the presence or absence of 1 X MEM amino acid solution (Gibco, CA, USA) with or without 100 M cystine
Knowing which tier you are looking at is the only way to make sense of the numbers
P.MadamwarD
The problem is that it breaks down in the stomach, and does not make it intact to the cells where it is needed
These either degrade the copper complex, strip the copper ion, or generate oxidative byproducts
