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Russo et al., AIDS, 2024)

Eluate incubated with TEV protease was dialysed against 25 mM Tris (pH 7.5), 150 mM NaCl and 1 mM DTT overnight at 4 C, then incubated with Ni-NTA resin for 1 h to remove the His-tagged protease

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As a result, GHK-Cu should be viewed as a well-tolerated adjunct with promising data rather than a proven equivalent to prescription retinoids

Interestingly, older female Nrf2-KO mice spontaneously develop a lupus-like phenotype 5 while the exact role of neutrophils in this context has not been investigated yet