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A previous study in macrophage cells also similarly showed that GSTO1 knockdown as well as GSTO1 inhibitor M175 (ref

doi: 10.1016/j.ijfoodmicro.2013.06.003 31 HuangK.NitinN

There was a significant inverse correlation between plasma GLP-1 concentration shortly after a meal and plasma glucose concentration 2 hours post-prandially, coinciding with the plasma glucose nadir

The cells were lysed in 25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1% -mercaptoethanol, 10 mM MgCl 2 , 2.1 mM leupeptin, 1.53 mM aprotinin and 50 U of benzonase (Novagen)