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Subsequently, we applied a focused refinement approach on the particles used in the reconstruction of holo-complex to improve the resolution for c-MET I and HGF I, resulting in a new cryo-EM map with resolution improved to 4.5 , which allowed us to build reliable models for the K3 and K4 domains of HGF (Supplementary Figs
IM sites have strict volume limits: deltoid maximum 1 mL, vastus lateralis 2 mL, ventrogluteal 3 mL
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