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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)
The subcutaneous tissue is well-vascularised enough to absorb the medication effectively whilst minimising discomfort and the risk of complications
The first phase (active weight loss) runs at higher doses and higher costs for 3-6 months
This number comes from preclinical research on dihydroberberine, the active form of berberine used in the product
Our DCF uses a 10% discount rate representing the acquiror's discount rate
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