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The electrospray was set in positive ionization mode with the following parameters: 30 psi for curtain gas supply, 500 C for the capillary temperature, 5500 V for the spray voltage floating, and 80 V for the declustering potential

J Biol Chem (2014) 289:1524458.10.1074/jbc.M113.540633 34 TurpaevKT

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An aliquot from the wells containing the most active LacAnc100 and LaccID clones was inoculated in 3 ml of YPD (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-glucose (Thermo Scientific, 410955000) and 25 g ml 1 chloramphenicol (Merck, C0378)) and incubated at 30 C and 225 rpm for 24 h

This is particularly relevant for individuals using cagrilintide alongside insulin or sulfonylureas