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N-acetylcysteine, or NAC, is a supplement that works in two ways: It provides cysteine, which is the rate-limiting amino acid for glutathione production It can also act as a direct antioxidant to scavenge ROS (NO2 and HOX)[ref][ref] The main antioxidant mechanism for NAC is by increasing glutathione levels

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In the gene knockdown and overexpression experiments, the corresponding empty vectors were used as the negative control in the transfection

Diabetic Retinopathy In patients with pre-existing diabetic retinopathy, rapid improvement in blood glucose control can temporarily worsen retinopathy