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A80-229AP, Bethyl Laboratories, Inc.), diluted in PBS/T/M (pH 5.5 or pH 7.4), was added for 1 h at room temperature, followed by a washing step and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)
One or more phospholipids can be used in a known manner to prepare liposomatic solutions
Under resting conditions, NF-B dimers are retained in the cytoplasm by inhibitory proteins such as IB
Shield, Soothe and Shine
MtROS are mobilized into the cytoplasm and then activate protein kinase C (PKC), subsequently activating NADPH oxidase and producing more ROS, which together with mtROS induces NETosis
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