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Without compound-specific stability testing, the cleaner research assumption is to use the shorter window, reconstitute only what is needed, and label the vial immediately

Anaphylactic reaction after injection of vitamin B12 Heres a case of two female patients with pernicious anemia who had adverse reactions to B12 shots (hydroxocobalamin)

In adults and older children, the total volume used should also stay within safe limits animal studies suggest up to about 30 mL (the full vial) can be given to an adult without benzyl-alcohol toxicity, but one should still use only the amount needed and discard the rest if sterility is lost

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Even with B12 in the diet, absorption depends on stomach acid and a protein called intrinsic factor, both of which decline with age and with certain medications