Respiratory Medicine Case Reports , 30 , 101063
For correcting a deficiency, the subcutaneous route is considered similarly effective to the intramuscular route
Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks
Because each spray delivers a fixed 50mcg + 50mcg dose, researchers can log intake precisely across a study timeline without weighing or measuring liquid volumes
Its typically only prescribed on a short-term basis