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All the WT-AIM interactions with DAMPs exhibit Ca 2+ concentration dependence, and mutating the two aspartic acid residues (D270A and D271A) abrogates the binding for all the cases, suggesting the indispensability of the first calcium site

The lack of relaxation effects by 3 M ACh in rings pre-contracted with 3 M PE confirmed the complete endothelium removal

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BDNF-Adjacent Pathways: While GHK-Cu does not directly upregulate BDNF (Brain-Derived Neurotrophic Factor), its anti-inflammatory effects in the CNS specifically its reduction of microglial activation may create conditions conducive to BDNF signaling