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After slices were dehydrated in progressively stronger alcohols, cleaned in xylene, and mounted, hematoxylin was used as a counterstain ( Measurement of the percentage area of a positive SIRT -1 immunoreaction in hepatic tissue Images were taken using a digital camera (Toucan type BX53, Japan) linked to a computer and a light microscope (Olympus model BX53, Tokyo, Japan)

Descriptive and inferential statistics were used to create heatmaps for visualizing changes in the concentration of each lipid analyte for every condition as previously described (Leishman et al., 2016)

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Biological Pathways Biological pathways are chains of chemical reactions inside cells that have specific results

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