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Fibroblast activation
The bound ligand was separated from free ligand by filtration through a Perkin Elmer Unifilter-96 GF/C 96-well microplate using the Perkin Elmer Unifilter-96 Harvester (Perkin Elmer, Waltham, MA), followed by three washes with ice-cold assay buffer
First, the peptide molecules begin to unfold from their precise three-dimensional configurations
This is especially important for those who may have difficulty absorbing nutrients due to digestive issues, certain medical conditions, or simply because of age-related changes
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