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Additionally, multi-modal theranostic nanoplatforms integrating imaging (MRI/fluorescence) and therapy will enable real-time monitoring of drug delivery and efficacy, facilitating adaptive treatment adjustments

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2.11 Reduced glutathione quantification GSH quantification was performed using established Ellman Assay, where GSH reacts specifically with the Ellmans reagent (DTNB) to yield 2-nitro-5-mercapto-benzoic acid (TNB), which produces a concentration-dependent yellow chromophore quantifiable by spectrophotometry ( 2.12 Measurement of myeloperoxidase activity Neutrophils are primary responders to inflammation, which can be studied by quantifying the activity of myeloperoxidase, an enzyme abundantly present in their granules ( 2.13 Histopathological examination Liver and pancreatic tissues were duly fixed in formalin (10%) solution to preserve structural integrity and prevent decomposition

Simultaneous folate transport deficits (e.g., FR autoantibodies, MTHFR polymorphisms) result in decreased methylation potential and glutathione imbalance