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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample

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Figure 3 At the initiation of oral semaglutide 3 mg, participants in the higher-dose groups, particularly those in the 14 mg group, were more likely to have been receiving two or three other oral antidiabetic drugs prior to initiation