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10.1016/j.neo.2017.10.005 Neoplasia 52 HombachS.KretzM

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Cells were incubated with serum-free media for 24 h prior to each experiment and seeded at 1 10 6 cells/dish in 100 mm culture dishes for western blotting and 3 10 4 cells/well in 24-well- culture plates for immunofluorescence

Similarly, if kidney function declines during treatmentwhether due to dehydration, illness, or age-related changesmetformin may need to be stopped because the kidneys cannot safely clear the medication

The appetite suppression directly opposes treatment goals and creates medical risks