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Plasma levels drop from approximately 200 ng/ml at age 20 to around 80 ng/ml by age 60
That suggests appetite suppression was not the main mechanism in those studies
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To test LaccID in a similar manner, we created an ER-targeted construct by appending a KDEL ER retention sequence and prepared analogous constructs for APEX2 and HRP for comparison
Sharer JE, Shipley LA, Vandenbranden MR, Binkley SN, Wrighton SA
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