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Freezing a reconstituted peptide solution can cause: Ice crystal formation that mechanically disrupts peptide structure Phase separation or precipitation upon thawing Reduced bioactivity in subsequent experiments Refrigerator storage maintains the peptide in a stable aqueous environment
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Ben Othman S, Katsuno N, Kitayama A, Fujimura M, Kitaguchi K, Yabe T
Instead, Cys497 hTrxR1 is sandwiched between Ser415 hTrxR1 and Ser111 hTrxR1 , the latter being within hydrogen bond distance (3.6 ) to a strictly conserved His108 hTrxR1 (Fig
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