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Statements Author contributions JnL and QZ conceived the study

In rats, GCLC has a Km for glutamate that is about 10-fold higher than that of the GCL holoenzyme, which is higher than the cellular glutamate concentration in most tissues GCL is specific for the glutamyl moiety and is regulated physiologically by: (a) non-allosteric feedback competitive inhibition by glutathione (GSH) (Ki = 2.3 mM) which involves binding of GSH to the glutamate and another site on the enzyme [8, 9] and (b) availability of its precursor, cysteine [1]

Two registered trials exist: a Phase 1 safety and pharmacokinetics study (NCT02637284) with no posted results, and a Phase 2 hamstring-strain trial (NCT07437547) still recruiting

The cells were then stained and subjected to confocal imaging

But you can also have a vitamin B12 deficiency without having anemia