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Clashes between protein and lipid atoms were reduced through 2500 conjugate-gradient minimization steps, followed by a 6 ns long MD simulation with a linearly-released positional constraint of 1 kcal mol 1 2 on protein and lipid phosphorus atoms
It doesn't have relevance to real-world training conditions, where people are not training fasted
In the meantime, recombinant AIM was diluted at 20 g/mL in blocking buffer with various concentration of CaCl 2 (0
With subsequent binding, it leads to increased fat metabolism
Aller, I., Rouhier, N
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