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Prevents bacterial growth for up to 28 days after first use

Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)

The DNA sequence encoding cleaved peptides could be identified by high-throughput sequencing of library members in surviving bacteria to quantify the relative activity of the protease on all substrates in the library via high-throughput sequencing

Since it is well known that both ubiquitination and phosphorylation are involved in NRF2 regulation, it was no surprise that the acetylation of NRF2 also enhances its activity [214]

These protocols also promote telomere elongation, reduce DNA damage, and assist in cellular adaptation