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Cancer cells are mutated, dysfunctional versions of healthy cells
Secondary antibodies (AlexaFluor 594 anti-mouse 1:500 (Invitrogen, A11005) and AlexaFluor 488 anti-rabbit 1:500 (Invitrogen, A11034)) were added to blocking buffer and incubated at room temperature for 1 h
10.1093/emboj/18.5.1321 EMBO J
From a clinical lens, determining the optimal ways to modulate the NRF2 pathway in stem cells, ex vivo for cell transplantation, or in directly in vivo through the development of effective small molecule inhibitors acting upstream or downstream of NRF2, will be critical
Following importation across the plasma membrane by system Xc-, cys is promptly converted to cysteine, which is considered the limiting precursor of GSH synthesis
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