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Continue until the solution appears completely clear with no visible powder

Sections were examined using a Hitachi H-7650 transmission electron microscope equipped with a charge-coupled device camera, operating at 80 kV

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Raleigh DR, Marchiando AM, Zhang Y, Shen L, Sasaki H, Wang Y, Long M, Turner JR: Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions