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Rats were anesthetized using inhalational anesthesia with a small animal anesthesia machine (RWD Inc., Shenzhen, China), inducing anesthesia with 3% isoflurane followed by maintenance at 1.5% isoflurane
As an example, GSH depletion has been shown to promote pro-inflammatory microglial phenotypes and amplify astrocyte-microglia crosstalk (Vilhardt et al., 2017)
Although one thought may be through the upregulation of neurotransmitters in which are directly correlated with Testosterone, specifically dopamine
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