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Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose
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Timing: Taking glutathione on an empty stomach, typically 30 minutes before meals or two hours after eating, can prevent competition with other nutrients, maximizing absorption
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