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To reduce the effector functions mediated by the Fc fragment, researchers converted the antibody subtype to a non-glycosylated mouse IgG1 subtype by introducing an N297 to G mutation to achieve deglycosylation

For molar calculations, use the verified net amount of free-peptide equivalent from the COA: Molar concentration = moles of free-peptide equivalent / final solution volume Do not calculate moles from total vial mass alone when the material contains water, a counterion, or other non-peptide components

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7B) combining and contributing to the 3890 nm peak