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For Plp-staining cells were washed and stained with anti-Plp 1:500 (NB100-1608) (Novous Biologicals, Littleton, CO) overnight followed by anti-chicken-Cy3 (Thermo Fisher Scientific, Waltham, MA) and co-stained with CellMask and Hoechst (Thermo Fisher Scientific, Waltham, MA) for 5 min at +4 C
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: Skin Glow , 2,33
Before we could continue our investigation, however, we wished to validate a published dopamine depletion model [25]
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