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The date of first use should be recorded on the vial, and the medication should be discarded after the specified period following initial puncture, typically 28 days, even if medication remains and the beyond-use date has not been reached

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Maquart 1988 J Clin Invest showed that GHK-Cu at nanomolar concentration stimulates type I collagen synthesis by fibroblasts by 60-200 %, of glycosaminoglycans (hyaluronic acid, heparan sulphate) and proteoglycans

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However, recent clinical evidence suggests the picture is more nuanced than previously understood