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Potential analytical variables include: Metal-binding affinity Stoichiometry Complex formation Free copper concentration Competition with other ligands Redox state Charge distribution Time-dependent dissociation A copper-binding observation does not establish delivery of copper to a specific tissue, cellular benefit, or therapeutic effect
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Ala16Val mutation in Mn-SOD changes the secondary structure of the protein, which may affect the targeting of the protein to mitochondria [63]
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