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The major molecules from each subfamily, which have the most significant impact on neutrophil chemotaxis, are as follows: N-formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe or fMLF), C-X-C chemokines, C5a, and leukotriene B 4 (LTB 4 )
The excitation (ET430/20x and ET500/20x, ET type, Chroma Technology Corporation) and emission (650/60 m) filters (BrightLine type, Semrock) were used in combination with an FF444/521/608-Di01 dichroic beamsplitter (Semrock) and reported as an excitation ratio (R430/500)
Buffer selection becomes critical for maintaining pH stability during storage and reconstitution processes
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