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Finally, donkey anti-rabbit Alexa 594 (711-586-152, Jackson) was added to the cell suspensions at 1:100 dilution, for 30 min
For pseudo-TIRFM, the calculation of the penetration depth was performed by measuring the position at which the beam was exiting from the center of the objective and subsequently correlated to the penetration depth versus the displacement of the beam from the center using the exponential decay depth equation described previously 73 , incorporated into the Nikon software
Use standard laboratory PPE and follow your facilitys SOPs for peptide reagents
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Instead, efforts were redirected to develop and assess an improved once-daily formulation aimed at enhancing the drug's tolerability and patient acceptance
