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Environmentally Relevant Concentrations of Microplastic Exposure Cause Cholestasis and Bile Acid Metabolism Dysregulation through a Gut-Liver Loop in Mice

At the end of this 7-day labelling period ( t = 0 h), samples were collected to verify complete incorporation of the heavy label into cellular proteins

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In vitro cultures Following 5-6 h of fertilization, oocytes were washed twice in Charles Rosenkrans (CR1aa) culture medium, containing 0.4 mM sodium pyruvate, 5 mM lactate, 5% calf serum, 20 g/mL streptomycin sulphate