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In brief, HCT116 cells were infected with the GeCKO v1 human CRISPR knockout pooled library using lentiviral transduction at a multiplicity of infection of 0.3, and populations stably expressing gRNAs were selected for using puromycin and expanded for 8 days to allow for essential gene drop-out

Step-by-Step Guide for Administering B12 Injections Administering B12 injections properly is essential for effective treatment and patient safety

1k, with AUC) as was food intake following the IPGTT and AL-fed blood glucose (Extended Data Fig

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