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Core Biological Themes Covered GLP-1 physiology hunger, insulin, glucose, fat metabolism Microbiome SCFAs GLP-1 axis why microbial metabolites matter Stress & cortisol loops how stress overrides satiety Metabolic flexibility mitochondria, fat oxidation, insulin sensitivity Circadian timing sleep, appetite rhythms, metabolic control
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Preparation of hypothalamic samples for LC-MS/MS analysis For the proteomic analysis by LC-MS/MS, hypothalamic samples were dissected, immediately frozen in liquid nitrogen, and stored at 80 C until used
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