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In a study investigating the mechanism of this effect, researchers used pancreatic tissue fragments from normal and diabetic rats

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Methods Insect cell expression HA-signal peptide-FLAG-3C-GLP-1R-3C-8HIS 13 , human DNG s 46 , His 6 -tagged human G 1 and G 2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described

The application method is the same