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Activity was measured on an Agilent 8453 UV/Vis spectrophotometer on kinetic mode (340 nm absorbance every 5 s for 275 s) upon the addition of 1.0-mM NAD + , 2.5-mM pyruvate, 200-M thiamine pyrophosphate, 100-M CoASH, and 5.0-mM MgCl 2
During positive selection, selection phage are modified to express the desired protease instead of gene III ( gIII ), a gene required for phage propagation 35 (Fig
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K., Maris, J
Optimizing healing during recovery becomes a priority
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