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Sequential additions of 0.1% trifluoroacetic acid (TFA) in acetonitrile and 0.1% TFA in 50% acetonitrile were added to the S-trap, centrifuged and pooled

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Canonical dynamics: equilibrium phase-space distributions

To solubilize melanin-associated proteins, material was resuspended in 50 l 2% SDS (end concentration) and incubate in ThermoMixer C (Eppendorf AG, Germany) at 55 C for 3 h