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One hundred microliters of a 50% slurry of prewashed protein G-agarose beads (GE Healthcare) was then added to each sample, followed by incubation for an additional 4 h at 4C

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103 However, Wnt3a is reported to be capable of activating transcriptional coactivator with PDZ-binding motif (TAZ), which can directly upregulate IRS-1, indicating its potential ability of increasing insulin sensitivity in muscle

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Trends Cancer 12 , 101104 (2026)